Figure 2
From: Beta-cell regeneration from vimentin+/MafB+ cells after STZ-induced extreme beta-cell ablation
Neogenic beta-cells survived and acquired functionality after insulin treatment.
(A) Representative islets stained with antibodies against insulin (red) and glucagon (green) of normal control SD rats (Nor), STZ-treated rats after 48 hrs (48 h), ins-treated rats after 7 days’ insulin treatment with a one-day washout (insulin-treated) and concurrent non-treated control rats (non-treated). Nuclei were labeled with DAPI. Scale bars, 100 μm. (B) Quantification of beta-cell number. (C) Fasting serum insulin levels in normal control SD rats (Nor), STZ-treated rats after 16 and 48 hrs (16 h, 48 h), insulin-treated rats by day 7 (insulin-treated) and concurrent non-treated control rats (non-treated). (D and E) Plasma glucose and insulin during an IP-GTT. (F) GSIS in vitro, measured as the ratio of insulin release in high glucose to insulin release in low glucose. Data are shown as means ± SEM, n = 6 rats per group; **P < 0.01.
