Figure 4

Neogenic beta-cells manifested vimentin expression.

(A) Photomicrographs double stained with anti-insulin (green) and anti-vimentin (red) antibodies from normal control rats (Nor) and STZ-treated rats at different time points (16 h, 24 h, 48 h). Nuclei were labeled with DAPI. Scale bars, 50 μm. The isosceles triangle: insulin⁺ cells co-expressing vimentin. The boxed regions showed the high power photomicrograph at the bottom right of the image. (B) Photomicrographs triple stained with anti-vimentin (red), anti-insulin (green) and anti-Ki67 (blue) antibodies. The isosceles triangle: vimentin⁺ cells co-expressing Ki67. The arrows: vimentin⁺/insulin⁺ cells showing negative Ki67 expression. Scale bars, 50 μm. (C) Quantification of insulin positive cells co-expressing vimentin. (D) Quantification of vimentin⁺ and vimentin⁺/insulin⁺ cells co^-expressing Ki67. Data are shown as means ± SEM, n = 5–6 per group; *P < 0.05 and **P < 0.01.