Figure 6

Round-shaped vimentin⁺ cells showed strong MafB expression.

(A) Photomicrographs double stained with anti-vimentin (red) and anti-glucagon (green) antibodies. Nuclei were labeled with DAPI. Scale bars, 50 μm. The isosceles triangle: round-shaped vimentin⁺ cells showing negative glucagon expression. (B) Quantification of glucagon-positive cells co-expressing vimentin. (C) Photomicrographs double stained with anti-vimentin (green) and anti-MafB (red) antibodies. Nuclei were labeled with DAPI. Scale bars, 50 μm. The isosceles triangle: round-shaped vimentin⁺ cells coexpressing MafB. The boxed regions showed the high powered photomicrograph at the bottom right of each image. (D) Quantification of vimentin-positive cells co-expressing MafB. (E) Photomicrographs triple stained with anti-vimentin (red), anti-insulin (green) and anti-MafB (blue) antibodies. Scale bars, 50 μm. The isosceles triangle: cells co-expressing vimentin, insulin and MafB. Data are shown as means ± SEM, n = 5–6 per group. *P < 0.05 and **P < 0.01.