Figure 4 | Scientific Reports

Figure 4

From: Herbal formula, Scutellariae radix and Rhei rhizoma attenuate dimethylnitrosamine-induced liver fibrosis in a rat model

Figure 4

Effect of SRE on the GPx (A) and SOD (B) levels in control sample and DMN-treated liver tissues. (C) The levels of Nrf-2 and catalase under various treatments were performed by Western blot analysis. The relative expression to GAPDH is shown at the bottom. (D) Effect of SRE on the MDA production. (E) Expression of protein carbonylation. Significantly increased levels of carbonylated proteins were observed in the DMN group compared to the control, while SRE could effectively reduce the levels of carbonylated proteins. The quantified ratios are shown by the bar chart. (F) 8-OHdG levels were measured by immunocytochemistry. 8-OHdG-positive cells (indicated by arrow) were increased in the DMN treatment group compared to the normal control group. SRE decreased 8-OHdG levels in hepatic cells under DMN-induced oxidative stress. The data are the means ± S.D. (n = 3) and * indicates a significant difference (p < 0.05). The intensity was calculated using the Image Pro-Plus 4.5 computer program. IOD: integrated optical density.

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