Figure 1 | Scientific Reports

Figure 1

From: Spinal muscular atrophy phenotype is ameliorated in human motor neurons by SMN increase via different novel RNA therapeutic approaches

Figure 1

Generation and characterization of SMA iPSC lines.

(A) Scheme of the iPSC generation protocol. Human iPSCs were generated by transducing human fibroblasts with retroviruses that encoded four reprogramming transcription factors (OCT4, SOX2, KLF4, and c-MYC). (B) When cultured under standard human ESC culture conditions, the morphology of human iPSCs is identical to that of human ESCs. The cells also express the pluripotency markers NANOG, SSEA3 and TRA-60-1 and demonstrate strong endogenous alkaline phosphatase activity (AP). Contrast phase images show the typical ESC morphology. Scale bar: 75 μm.

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