Figure 1 | Scientific Reports

Figure 1

From: INC280, an orally available small molecule inhibitor of c-MET, reduces migration and adhesion in ovarian cancer cell models

Figure 1

INC280 inhibits HGF-stimulated c-MET phosphorylation and downstream signalling.

3 cell lines (SKOV3, OVCAR3 and CaOV3) were treated with HGF, HGF + INC280, or left untreated, for 10 min (A), 360 min (B), or 24 hrs (D). c-MET phosphorylation was assessed in these cell lines using antibodies against phosphorylated Y1234/1235 c-MET (P c-MET) and total c-MET (T c-MET). C) Signaling downstream of c-MET activation was assessed (shown here 360 minutes post treatment using antibodies against phosphorylated S473 AKT (P AKT), total AKT (T AKT), phosphorylated Y202/204 ERK 1/2 (P ERK) and total ERK. β-actin was used as a loading control. HOSE 6.3 normal ovarian surface epithelium cells were also treated with HGF, HGF + INC280, or left untreated, for 10 min (E), 360 min (F) or 24 hrs (G). Full length gels containing the cropped images above are shown in Supplementary Figures 4 (A and B), 5 (C) and 6 (D–G).

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