Figure 3

RalB regulates dissemination of TGFβ-treated cells.

(A) RalB silencing impairs TGFβ-induced dissemination in CIA. TGFβ-treated cells were depleted of RalA or RalB, submitted to CIA and the speed of dissemination was evaluated by manual tracking. Number of cells from at least three experiments: siControl n = 76, siRalA-I n = 66, siRalA-II n = 44, siRalB-107 n = 30, siRalB-1749 n = 13, siRalB-333 n = 28. (B) Validation of Ral proteins depletion. Efficiency of RalA and RalB depletion was verified by western blotting.(C) Effect of Ral depletion on TGFβ-induced contractility. Representative images showing gel contraction by TGFβ-treated cells depleted of RalA or RalB. (D) Quantification of gel contraction upon Ral depletion. Mean percentage values from four to five experiments were plotted on graphic. Error bars represent SEM. p values come from two-tailed Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001.