Figure 5
Stimulation of EphB2 induces tau dephosphorylation with activation of PI3K/Akt and inhibition of GSK-3 in hippocampus of human tau transgenic mice.
EphrinB1/Fc or Fc was infused stereotactically into the hippocampal CA3 region of the human tau transgenic mice for 45 min and then the total and the activity-dependent phosphorylation levels of PI3K (a, b), Akt (c, d), GSK-3 (e, f) and the phosphorylation level of tau (g, h) were measured by Western blotting. The phosphorylation level of the kinases was normalized against total kinase level, while the phosphorylation level of tau was normalized against total tau probed by R134d. The total levels of the kinases and tau were normalized against tubulin probed by DM1A. The blots were representative of at least three independent experiments from at least 6 mice for each group and presented as the mean ± SD. *P < 0.05, **P < 0.01 versus Fc.
