Figure 3
From: A Novel Chimeric Anti-PA Neutralizing Antibody for Postexposure Prophylaxis and Treatment of Anthrax
Immunoprecipitation (IP).
A. 10% SDS-PAGE of IP. B. Western blot of IP. M, molecular weight marker (NEB, USA); lane 1, eluate of hmPA6 + a mixture of PA63 and PA83 from protein-A Sepharose; lane 2, hmPA6; lane 3, PA83; lane 4, eluate of anti-TLR4 chimeric IgG + a mixture of PA63 and PA83 from protein-A Sepharose. C and D. MS spectra of fragment ions from the 63 kDa protein. Five major (m/z = 870.539, 1267.709, 1479.797, 2034.010 and 2190.020) ions were detected. MS with protein database search: matched peptides are shown in red and boldface.
