Figure 6
From: Controlled electromechanical cell stimulation on-a-chip
Gene expression analysis.
A) Assessment of RNA qualities and concentrations using the Agilent Bioanalyzer RNA 6000 Pico Kit. RNA concentrations showed in the electropherograms varied from ~800 pg/μl to ~2 ng/μl, with an integrity number of 8.9 ± 0.1 representing an high quality RNA. (B) Agilent Bioanalyzer gel image of total RNA. (C) Histogram showing fold-changes in the expression of the selected genes following mechanical stimulation (7% strain), electrical stimulations (5 V/cm) and electromechanical stimulation (strain 7%, electrical signal 5 V/cm) compared to a control configuration without stimulation. GAPDH was chosen as housekeeping gene. Data are expressed as means ± SD.
