Figure 2
Effects of different doses of ROS scavengers on ROS level and Egr-1 protein expression in H9c2 cells after H/R, as assessed using flow cytometry and western blotting.
A. ROS levels; n = 6. B. Cropped blots show protein levels of Egr-1 and β-actin; n = 3. The bands were excised from the same gel. Data are expressed as the percentages of the control or H/R groups. All values are expressed as mean ± S.E.M. *P < 0.05 vs. control; #P < 0.05 vs. H/R; †P < 0.05 vs. H/R + 2 × 10−6 M EDA; §P < 0.05 vs. H/R + 2 × 10−5 M EDA; &P < 0.05 vs. H/R + 5 × 10−4 M NAC; *P < 0.05 vs. H/R + 2 × 10−3 M NAC.
