Figure 6
Influence of a ROS donor and JNK activator on the effects of F2 on ROS level, JNK activation and Egr-1 expression in H9c2 cells after H/R, as assessed using flow cytometry and western blotting.
A. ROS levels; n = 6. B. Cropped blots show total and p-JNK expressions; n = 3. The bands were excised from different gels which were run under the same electrophoresis condition. C. Cropped blots show Egr-1 and β-actin expressions; n = 3. The bands were excised from the same gel. Data are expressed as percentages of the levels of the control or H/R groups. All values are expressed as means ± S.E.M. *P < 0.05 vs. control; #P < 0.05 vs. H/R; †P < 0.05 vs. H/R + 10−6 M F2.
