Figure 1
From: High-resolution NMR characterization of low abundance oligomers of amyloid-β without purification
Biophysical characterization of Aβ1-40 disordered oligomers.
(a) CD spectra, (b) ThT fluorescence and (c) bis-ANS fluorescence of fibrillar (black), the spin-X-isolated oligomer (blue) and freshly dissolved (red) samples of Aβ1-40. In panel c, the emission spectrum of bis-ANS in solution is shown in grey. (d) Distributions of the hydrodynamic radii of the freshly dissolved Aβ1-40 (red) and the spin-X-isolated Aβ1-40 oligomer (blue) determined by DLS. Representative AFM and TEM images of the Aβ1-40 oligomers (e) and fibrils (f). Scale bars in both images are 100 nm. All experiments were performed in 10 mM sodium phosphate buffer, pH 7.4 at 25 °C.
