Figure 3
From: High-resolution NMR characterization of low abundance oligomers of amyloid-β without purification
MAS spectra of the filtered disordered Aβ1-40 oligomer.
An overlay of the assigned Hα regions from RFDR-based 2D 1H/1H (blue) and 2D 1H/1H TOCSY (red) spectra acquired at 298 K with 10 kHz MAS. The filtered oligomer sample was lyophilized and re-hydrated to double its initial concentration, making for a total Aβ1-40 of ~35 μM. The RFDR and TOCSY based 2D 1H/1H spectra were acquired with mixing times of 50 and 70 ms, respectively, at 600 MHz in 100% D2O, 10 mM sodium phosphate buffer, pH 7.4. RFDR spectra were acquired at 25 °C under 10 kHz MAS. Assignments are given for the RFDR-based 2D 1H/1H spectra. The non-uniform sampling based data acquisition time was 4 hours.
