Figure 1
From: A novel long non-coding natural antisense RNA is a negative regulator of Nos1 gene expression
Nos1-related natural antisense RNA (Mm-antiNos1 RNA) is expressed in the mouse brain.
In 1, Northern blot analysis of poly(A)+ RNA extracted from the mouse brain using the ‘sense’ probe. The ‘sense’ probe has the same sequence as Nos1 mRNA. A prominent band (shown by arrow) indicates the presence of endogenous antisense transcript of approximately 1500 nt. In 2, The result of Northern blot hybridisation with the ‘antisense’ probe that is complementary to Nos1 mRNA. As expected, a band of about 9 kb is revealed.
