Figure 2
From: A novel long non-coding natural antisense RNA is a negative regulator of Nos1 gene expression
Mm-antiNos1 RNA is a polyadenylated cis-encoded natural antisense transcript.
(a) Nucleotide sequence of Mm-antiNos1 RNA. The antisense region is shaded. A putative polyadenylation signal is underlined. The alternatively spliced exon is shown in grey letters. (b) The results of RT-PCR experiment performed on poly(A)+ RNA extracted from the mouse brain. A product of the expected size (about 0.45 kb) is produced when primer 1 was used in the reverse transcription reaction (lane 1). Note that there is no product in the experiment in which primer 2 was used (lane 2). (c) A schematic diagram showing that Mm-antiNos1 RNA is transcribed from the non-template strand of the Nos1 locus.
