Figure 3
From: A novel long non-coding natural antisense RNA is a negative regulator of Nos1 gene expression
Mm-antiNos1 RNA down-regulates Nos1 protein synthesis.
(a) Representative Western blot analysis of cultured Neuro2a cells incubated with transfection mixtures containing either 0.2 μg (lane 1) or 1 μg (lane 2) of pcDNA 3.1/Mm-antiNos1. Non-transfected control cells are shown in lane C. (b) Quantitative densitometric analysis of the Western blot shown in a. Values for the Nos1 expression were quantified relative to the expression of β-actin. Finally, the values for Nos1 expression in transfected Neuro2a cells were normalised to Nos1 protein levels in non-transfected cells.
