Table 1 Sample information.
From: Pros and cons of methylation-based enrichment methods for ancient DNA
Genus species | Location | Age (years Before Present) | ID | Tissue | Volume of extract captured(μl) | Total extract volume (μl) | PCR cycles | Lab |
|---|---|---|---|---|---|---|---|---|
Ursus maritimus | Zhokhov Island, Russia | ~8,000 | PB9 | bone | 25 | 200 | MBD−: 12, MBD+ : 12 + 10 | 1 |
Ursus maritimus | Zhokhov Island, Russia | ~8,000 | PB25 | bone | 25 | 200 | MBD−: 12, MBD+ : 12 + 10 | 1 |
Ursus maritimus | Zhokhov Island, Russia | ~8,000 | PB44 | bone | 50 | 200 | MBD−: 12, MBD+: 12 + 10 | 1 |
Equus ferus | Ice cave museum, Khatanga, Taymyr, Russia | 22,887 ± 125 | EQ26 | bone | 21.25 | 150 | MBD−: 9 + 8, MBD+ : 12 + 11 | 1 |
Equus ferus | Ice cave museum, Khatanga, Taymyr, Russia | 27,968 ± 213 | EQ34 | bone | 21.25 | 150 | MBD−: 9 + 9, MBD+ : 12 + 12 | 1 |
Equus ferus | Ice cave museum, Khatanga, Taymyr, Russia | 21,477 ± 94 | EQ36 | bone | 21.25 | 150 | MBD−: 9 + 9, MBD+ : 12 + 12 | 1 |
Equus lambei | Yukon Territory, Canada | Late Wisconsinian (11,000−25,000) | EQA | bone | 21.25 | 150 | MBD−: 10 + 8, MBD+ : 13 + 10 | 1 |
Hippidion sp. | Quebrada Opache, Chile | Late Pleistocene | EQB | bone | 21.25 | 150 | MBD−: 10 + 8, MBD+ : 13 + 10 | 1 |
Hippidion saldiasi | Betecsa-1, Chile | 21,070 ± 100, 21,380 ± 100 | EQC | bone | 21.25 | 150 | MBD−: 10 + 8, MBD+ : 13 + 10 | 1 |
Equus (Amerhippus) sp. | GNL Quintero 1, Chile | 24,890 ± 70 (bioapatite) | EQE | bone | 21.25 | 150 | MBD−: 10 + 8, MBD+ : 13 + 10 | 1 |
Hippidion sp. | Baño Nuevo-I, Chile | 13,275±30 | EQF | bone | 21.25 | 150 | MBD−: 10 + 8, MBD+ : 13 + 10 | 1 |
Mammuthus primigenius | Yenisei River Gulf, Taymyr Peninsula, Western Siberia | 44,750 (+950/−700) | MZh_1 | heart | 21.25 | 150 | MBD−: 12 + 8, MBD+ : 14 + 11 | 1 |
MZh_2 | heart | 21.25 | 150 | MBD−: 8 + 6, MBD+ : 12 + 9 | 2 | |||
MZl_1 | liver | 21.25 | 150 | MBD−: 12 + 8, MBD+ : 14 + 11 | 1 | |||
MZl_2 | liver | 21.25 | 150 | MBD−: 8 + 6, MBD+ : 12 + 9 | 2 | |||
MZm_1 | skeletal muscle | 21.25 | 150 | MBD−: 12 + 8, MBD+ : 14 + 11 | 1 | |||
MZm_2 | skeletal muscle | 21.25 | 150 | MBD−: 8 + 6, MBD+ : 12 + 9 | 2 | |||
Mammuthus primigenius | Khroma River, Yana-Indigirka lowland, Yakutia, Eastern Siberia | ≥45,000 | MKm_1 | skeletal muscle | 21.25 | 150 | MBD−: 12 + 8, MBD+ : 14 + 11 | 1 |
MKm_2 | skeletal muscle | 21.25 | 150 | MBD−: 8 + 6, MBD+ : 12 + 9 | 2 | |||
MKs_1 | skin | 21.25 | 150 | MBD−: 12 + 8, MBD+ : 14 + 11 | 1 | |||
MKs_2 | skin | 21.25 | 150 | MBD−: 8 + 6, MBD+ : 12 + 9 | 2 | |||
MKhs_1 | hair and skin | 21.25 | 150 | MBD−: 12 + 8, MBD+ : 14 + 11 | 1 | |||
MKhs_2 | hair and skin | 21.25 | 150 | MBD−: 8 + 6, MBD+ : 12 + 9 | 2 | |||
MKb1_1 | bone 1 | 21.25 | 150 | MBD−: 12 + 8, MBD+ : 14 + 11 | 1 | |||
MKb1_2 | bone 1 | 21.25 | 150 | MBD−: 8 + 6, MBD+ : 12 + 9 | 2 | |||
MKb2_1 | bone 2 | 21.25 | 150 | MBD−: 12 + 8, MBD+ : 14 + 11 | 1 | |||
MKb2_2 | bone 2 | 21.25 | 150 | MBD−: 8+6, MBD+ : 12 + 9 | 2 | |||
Homo sapiens | Qeqertasussuk, Greenland | 4,044 ± 31 | HSSR | hair | 70 | 80 | MBD−: 8/10, MBD+ : 12 + 10/15 + 7 | 1 |
