Figure 1

Diagrammatic illustration of our method for characterization of 13C-labeled plant metabolites and lignocellulose.
(a) Intact sample and DMSO/pyridine extraction of 13C-labeled poplar were used for HR-MAS and solution-state NMR, respectively. (b) Signal assignments of metabolites and lignocellulose components could be achieved by 3D HCCH-COSY and HCCH-TOCSY. (c) Based on signal assignments of lignocellulose components, application to two transgenic 13C-poplar samples were compared with wild-type poplar.