Figure 3 | Scientific Reports

Figure 3

From: Strategy to enhance efficacy of doxorubicin in solid tumor cells by methyl-β-cyclodextrin: Involvement of p53 and Fas receptor ligand complex

Figure 3

MCD potentiates DOX-induced death of MCF-7 and Hepa1–6 cells in a p53-dependent manner by upregulating FasR/FasL.

MCF-7 and Hepa1–6 cells were treated with indicated concentration of DOX together with MCD for 24 h. (A) Protein levels of p53 and MDM2 were examined by western blotting analysis. Hsp60 served as internal control was used a loading control. (B) MCF-7 and (C) Hepa1–6 cells were processed for immunostaining to detect p53 expression and its nuclear localization by immunofluorescence confocal microscopy. (D) FasR membrane staining was performed by flow cytometry. (E) Protein levels of FasR and FasL were examined by western blotting analysis and Hsp60 was used a loading control. (F) Sandwich ELISA for quantitation of secreted FasL from culture medium of MCF-7 and Hepa1–6 cells. Bar graph represents the Mean ± SD of an experiment done in triplicate. Cropped blots are used in the main figure and full length blots are included in Supplementary figure 3.

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