Figure 3

Phylogenetic tree indicating inferred relatedness of ppk1 genes from Accumulibacter by employing maximum likelihood method.
The sequences produced by ppk1 gene clone libraries of CN-GZ-DT sludge sample were colored by green and those of JP-A2O-TK sludge sample were colored by blue. The sequences in red are derived from draft genome sequences rather than clones. Black circles on node branches indicate >60% bootstrap support. The ppk1 gene of Rhodocyclus tenuis was adopted as the most closely related outgroup sequence.