Figure 4
From: Structural insights into the unique inhibitory mechanism of the silkworm protease inhibitor serpin18
Expression patterns and localization of serpins in the silk gland.
(A) Expression patterns of serpin16, serpin18 and serpin22 genes in the day1, 3, 5, 7 of the fifth instar (V-1d, V-3d, V-5d, V-7d) and the hour 12 after wandering (W-1d). (B) Expression patterns of serpin were from female or male larvae in the different segments of the silk gland in the day 5 of the fifth instar. Western blot analysis for serpin18 and its paralogs in the (C) developmental stages of the fifth instar and (D) different segments of the silk gland. The silkworm tubulin was used as internal control. The gels were run under the same experimental conditions and the full length blots and gels are presented in Supplementary Figure S6. (E) Immunofluorescence analysis of serpin18 and its paralogs in the A-MSG on fifth day of the fifth instar. Slides were incubated with anti-serpin16 antibody followed by the secondary antibody labeled with FITC (greeen) and counterstained with DAPI (blue). Control experiments were also performed using pre-immune serum. Bar, 100 μm.
