Figure 1 | Scientific Reports

Figure 1

From: Formation and manipulation of cell spheroids using a density adjusted PEG/DEX aqueous two phase system

Figure 1

ATPSs consist of 35 K PEG and 500K DEX.

(A) Experimentally-acquired phase diagram of 35 K PEG and 500 K DEX in PBS. Points above the curve can form two immiscible liquid phases whereas points below the curve cannot. The bottom phase is DEX-rich; the top phase is PEG-rich phase. Formation of the two phases can be easily visualized using colored food dye which is preferentially partitioned to one phase (here, blue food dye partitioned to the PEG-rich phase). (B) DEX-in-PEG ATPS pattern. Due to its immiscible nature, DEX-rich phase can be patterned inside the PEG-rich phase. Successful pattern showed a clear circular PEG-DEX interface and was stable for ~5 d.

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