Figure 4 | Scientific Reports

Figure 4

From: Formation and manipulation of cell spheroids using a density adjusted PEG/DEX aqueous two phase system

Figure 4

Spheroids of various cell lines formed using DEX-in-PEG ATPS and HD method.

Well-known spheroid forming cells (ES D3, NIH-3T3, MCF-7 and HCT 116) showed regular-shaped and tight cell spheroids 48 h after ATPS patterning. Non-spheroid forming cell (MDA-MB-231) and slow-spheroid forming cell (HepG2) cell showed loose cell aggregates and irregularly-shaped aggregates respectively. Cells cultured with HD method showed similar but less effective spheroid forming capacity in terms of sphericity and tightness. Scale bars: 400 μm.

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