Figure 5
Size consistency and size controllability of spheroid formation using DEX-in-PEG ATPS pattern.
(A) Twenty-four spheroids of ~5,000 HCT 116 cells from 24 DEX-in-PEG ATPS pattern in 96-well plate. The size and shape of the spheroids seemed consistent under a phase contrast microscope. Scale bar: 200 μm. (B) Measured Feret’s diameters of 24 HCT 116 spheroids. Spheroids had average diameter 347.9 μm (solid horizontal line) with standard deviation 23.0 μm (dotted lines, mean ± one standard deviation); 20 of the 24 spheroids were included in this range. (C) Phase contrast microscope images of four spheroids for each group (5000, 2500, 1250, 625, 312 and 156 HCT 116 cell spheroids) were generated using DEX-in-PEG ATPS patterns in a 96-well plate. Size increased cell number increased. Scale bar: 200 μm. (D) Feret’s diameter (y) vs. number of cells (x). Regression, ln(y) = 0.330∙ln(x) + 2.90, r2 = 0.996 (bars: ±1 sd, n = 6).
