Table 2 Stability data of fructose-based 3-acetyl-2,3-dihydro-1,3,4-oxadiazole (GLB) detected by high performance liquid chromatography (HPLC) method in rat plasma (Mean ± SD, n = 3).
Stability | Actual concentration (μg/mL) | Measured concentration (μg/mL) | Precision (RSD, %) | Accuracy (RE, %) |
|---|---|---|---|---|
Light and room temperature for 24 hours | 0.025 | 0.026 ± 0.002 | 7.52 | 4.17 |
0.5 | 0.486 ± 0.027 | 5.53 | −2.79 | |
4 | 3.922 ± 0.055 | 1.41 | −1.94 | |
Post-preparative stability (at 4 °C for 48 hours) | 0.025 | 0.026 ± 0.001 | 3.63 | 5.31 |
0.5 | 0.528±0.007 | 1.25 | 5.62 | |
4 | 4.233 ± 0.196 | 4.64 | 5.83 | |
Post-preparative stability (at 4 °C for 1 week) | 0.025 | 0.026 ± 0.001 | 4.19 | 3.83 |
0.5 | 0.428 ± 0.010 | 2.25 | −14.4 | |
4 | 3.668 ± 0.219 | 5.98 | −8.30 | |
Freeze-thawing 3 cycles (at −20 °C) | 0.025 | 0.025 ± 0.001 | −0.75 | 2.82 |
0.5 | 0.467 ± 0.036 | −6.51 | 7.65 | |
4 | 3.732 ± 0.227 | −6.71 | 6.08 | |
Freezing storage stability (at −20 °C for 2 weeks) | 0.025 | 0.027 ± 0.001 | 4.09 | 8.17 |
0.5 | 0.535 ± 0.009 | 1.68 | 7.10 | |
4 | 4.393 ± 0.090 | 2.04 | 9.84 | |
Freezing storage stability (at −20 °C for 1 month) | 0.025 | 0.027 ± 0.002 | 8.29 | 8.66 |
0.5 | 0.490 ± 0.015 | 3.01 | 1.17 | |
4 | 4.097 ± 0.213 | 5.19 | −2.27 | |
Freezing storage stability (at −20 °C for 3 months) | 0.025 | 0.023 ± 0.002 | −7.12 | 8.04 |
0.5 | 0.487 ± 0.003 | −2.66 | −2.04 | |
4 | 3.918 ± 0.063 | 0.71 | 1.62 |
