Figure 2 | Scientific Reports

Figure 2

From: miR-125a-3p and miR-483-5p promote adipogenesis via suppressing the RhoA/ROCK1/ERK1/2 pathway in multiple symmetric lipomatosis

Figure 2

miR-125a-3p and miR-483-5p regulate adipogenesis in hADSCs.

hADSCs were isolated from the abdominal SAT of two control patient respectively and we repeated all experiments by two kinds of hADSCs. The hADSCs were transfected with the miR-125a-3p or miR-483-5p agomir, agomir-NC (100 nM), antagomir-NC, or antagomir (200 nM), respectively. After 72 h, (A) The hADSCs were induced differentiation for 12 days, lipid droplet accumulation stained by Oil red O was observed under a microscope. (B) The hADSCs were induced differentiation for 12 days, lipid droplet accumulation stained by Oil red O was observed by a camera (no magnification). (C) The hADSCs underwent self-differentiation for 12 days, lipid droplet accumulation stained by Oil red O was observed under a microscope. (D,E) The hADSCs were induced differentiation for 12 days, the protein levels of PPARγ, C/EBPα and FABP4 were detected by Western blot. β-Actin served as a loading control. All measurements were preformed by three independent experiments.

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