Figure 4 | Scientific Reports

Figure 4

From: miR-125a-3p and miR-483-5p promote adipogenesis via suppressing the RhoA/ROCK1/ERK1/2 pathway in multiple symmetric lipomatosis

Figure 4

The expression of miR-125a-3p and miR-483-5p are increased with the induction of hADSCs.

(A) The hADSCs were induced to mature adipocytes for 12 days. The miR-125a-3p and miR-483-5p expression levels were detected by quantitative real-time PCR at days 0, 6 and 12. (B) The hADSCs were induced to mature adipocytes for 12 days. Total protein was extracted for immunoblotting of RhoA, ROCK1, total ERK1/2 (T-EKR1/2) and phosphorylated ERK1/2 (T-p-EKR1/2) by western blot. (C) The expression of RhoA, ROCK1, T-EKR1/2 and T-p-EKR1/2 in the adipose tissues of MSL patients (n = 3) and controls (n = 3) were determined by western blot. *p < 0.05, **p < 0.01 in comparison to relative expression levels at day 0.

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