Figure 1

qRT-PCR, western blot and immunohistochemistry analyses of RIPK4.
(a) qRT-PCR analysis of RIPK4 mRNA expression in 101 fresh CSCC tissues (T) and 30 paracancerous tissues (N). Expression levels were normalized to those of EFF1A1. (b) RIPK4 protein levels were determined by western blot in 8-paired CSCC tissues (T) and paracancerous tissues (N). GAPDH was used as a loading control. (c) Quantitative analysis of RIPK4 protein levels by western blot was presented as median ± interquartile. (d) RIPK4 immunohistochemical staining in representative cases from each cervical lesion. The scale bar represents 50 μm. (e) Expression of RIPK4 in each cervical lesion based on immunohistochemical scoring, as determined by the percentage of positive cells with strong staining. Values are the median with interquartile range.*P < 0.05 by the Wilcoxon rank-sum test.