Figure 3
From: Involvement of PrPC in kainate-induced excitotoxicity in several mouse strains
Chemical release of PrPC from the cell surface impairs the neuroprotective function in KA-treated N2a cells.
(a) Example of western blot determination of PrPC levels in protein extracts of pcDNA, pcDNA-PrPC, pcDNA-PrPC + PLC and pcDNA-PrPC + Gli treated N2a cells. (b) Microphotographs illustrating PrPC levels in cells transfected with pcDNA or pcDNA-PrPC and treated with PLC (pcDNA-PrPC + PLC) or Gli (pcDNA-PrPC + Gli). PrPC (green) was detected in non-permeabilized cells with 6H4 antibody. Note the decrease in PrPC after PLC and Gli treatments in (a) and (b). (c) Histogram showing the decrease in surviving cells after KA-treatment (5 mM overnight) determined with the WST-1 viability test after PLC or Gli treatment of pcDNA-PrPC transfected cells. Data (mean ± S.E.M.) were obtained from three independent experiments and represented as mean fold change. Scale bars represent 100 μm. Asterisks indicate statistical significance (**P < 0.05, Bonferroni post hoc test).
