Figure 2 | Scientific Reports

Figure 2

From: Promoter-level expression clustering identifies time development of transcriptional regulatory cascades initiated by ErbB receptors in breast cancer cells

Figure 2

Characterization of SRF, cFOS, cJUN, FOSL1, RARAlpha and DUSP5 genes at expression and protein levels.

(a) CAGE analysis captures the quantitative differences in transcriptional changes induced by EGF (red)- and HRG (black)-stimulated signaling pathways in MCF-7 cells. (b) Western blot analysis was performed to quantify protein levels after the stimulation of the same ligands (10 nM). (c) Western blot analysis was performed to quantify protein phosphorylation levels after the stimulation of the same ligands (10 nM). Western blot experiments were performed twice. Each dot in panels b and c represents the quantified values in an independent experiment and lines represent the average of these values.

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