Figure 1 | Scientific Reports

Figure 1

From: DNA-dependent targeting of cell nuclei by a lupus autoantibody

Figure 1

3E10 scFv penetrates most efficiently into living cells surrounding a dead cell.

GM02605 fibroblasts were washed with serum free media and then treated with 10 μM 3E10 scFv for one hour, followed by anti-Myc immunostaining to detect nuclear penetration by 3E10 scFv. Nuclear penetration by 3E10 scFv was restricted to cells in close proximity to what morphologically appears to be a dead cell, suggesting that a factor released by dead cells promotes nuclear uptake of the fragment.

Back to article page