Figure 2

Lipid and protein patterns in testicular LDs
A. Thin-layer chromatography analysis of total lipids extracted from LD preparations of mice testis, brown adipose tissue (BAT) and Chinese hamster ovary (CHO) cells. TG, triacylglycerols; CE, cholesteryl esters; EL, ether lipid. B. Silver-stained SDS-PAGE gels of protein extracts of different testicular lipid droplet preparations. C. Coomassie Blue-stained SDS-PAGE gels of proteins extracted from fractions of testicular LD, total membrane (TM), cytosol (Cyto) and post-nuclear supernatant (PNS). For the whole proteome, the lane running the testicular LD proteins was excised into 23 gel slices and underwent mass spectrometry.