Figure 2
The stage-dependent effect of minocycline (mino) in the object recognition test (A) and the Morris water maze test (B) under different regimens of administration (D0-32, D0-3 or D4-32) was assessed from 27 to 32 days after rUCCAO. Mice were then sacrificed at 32 days after rUCCAO for immunohistochemistry and electron microscopy examination. The MBP expression (C: immunostaining; D: western blot) and axonal damage (E: NF-200 immunostaining) was assessed. The percentage of thick (a in rUCCAO image), thin myelinated axons (b in rUCCAO image) or unmyelinated (c in rUCCAO image) were quantified from electron microscopy. (C,E) scale bar, 50 μm; (F) scale bar, 1 μm. A, B: n = 9–12; C, E: n = 6–8; D: n = 4–5; F: n = 3. Values are represented as mean ± s.d. ###P < 0.001, vs. the sham group; *P < 0.05, **P < 0.01, ***P < 0.001, vs. the rUCCAO group.
