Figure 3
The activation of microglia (B; Iba-1 staining) after minocycline (mino), MIF and LPS treatment was examined at 3 days after rUCCAO, with representative photographs shown in (A). The MBP protein expression (C: representative photomicrographs, D: quantitative analysis) and the cognitive ability of mice tested with the object recognition test (E) and the Morris water maze test (F), after minocycline (D0-3), MIF and LPS treatment were evaluated at 32 days after rUCCAO. Scale bar, 50 μm. B, D: n = 6–8; E, F: n = 7–8. Values are represented as mean ± s.d. #P < 0.05, ##P < 0.01, ###P < 0.001, vs. the sham group; *P < 0.05, **P < 0.01, ***P < 0.001, vs. the rUCCAO group; & & & P < 0.001, vs. the mino group.
