Figure 4
The total oligodendrocytes (Olig2+), OPCs (NG2+) and mature oligodendrocytes (CC1+) after minocycline (mino) D0-3 treatment were calculated as the percentage of the total cells (labeled by DAPI) at 3 days after rUCCAO (B), with the representative photomicrographs in (A) (the cells with arrows or arrowhead were enlarged in insets). Western blot analysis of NG2+, PDGFRα+ and Olig2 was also performed at 3 days after rUCCAO (C). The apoptosis of mature oligodendrocytes was analyzed by TUNEL staining, with representative photomicrographs in (D) and the quantitative analyses in (E and F), which were calculated as the percentage of the total cells (labeled by DAPI). Scale bar: 50 μm in A and 25 μm in C. A, B: n = 6–8; E, F: n = 4. Values are represented as mean ± s.d. ###P < 0.001, vs. the sham group; *P < 0.05, **P < 0.01, ***P < 0.001, vs. the rUCCAO group.
