Figure 5
The action of minocycline (mino) on OPCs proliferation in SVZ was determined after rUCCAO.
A cartoon depiction shows the analyzed SVZ region (red line) enlarged from the region of a mouse brain with dotted line (B). The Ki 67 + cells in SVZ were calculated as the percentage of the total cells (labeled by DAPI, C), with representative photomicrographs in A. The BrdU + NG2 + double positive cells (arrows in D) in SVZ were counted (E). The areas outlined by white dash line in F were SVZ areas and the SVZ areas with green outline were enlarged in the bottom lanes, while the corpus callosum (CC) areas with blue outline were enlarged in the right lanes. The retrovirus infected (GFP+) and NG2 + double positive OPCs (arrows in F) in SVZ areas shown in the bottom lanes and in CC areas shown in the right lanes were counted (G,H, the cells with hollow arrows in F are enlarged in insets). (A,D): scale bar, 20 μm; (F): scale bar, 50 μm. (C,E): n = 5–6; (G,H): n = 4. Values are represented as mean ± s.d. #P < 0.05, ##P < 0.01, vs. the sham group; *P < 0.05, *P < 0.01, vs. the rUCCAO group.
