Figure 6
The action of minocycline (mino) on OPCs proliferation was determined in cultured OPCs.
The MTT assay was performed after minocycline treatment (A). BrdU + cells were calculated as the percentage of NG2 + cells (B) and Ki 67 + cells were calculated as the percentage of total cells (labeled by DAPI, C), with the representative photomicrographs in (D) (the cells with arrows or arrowhead were enlarged in insets). The effect of minocycline (mino, 10 μM) on the cell cycle in cultured OPCs was determined by flow cytometry (E), with representative histogram shown in (F and G). Populations in the G0/G1, S and G2/M phases are shaded in yellow, red and blue, respectively. Scale bar, 50 μm. Values are from 3 to 4 independent experiments and represented as mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001, vs. the control group.
