Table 1 Cell physical parameters, methods and measurements.
Cell Physical Parameter | Method | Measurement |
|---|---|---|
Morphology | ||
Cell shape and size | Manual segmentation of brightfield images | Cell area |
Scanning Electron Microscopy | Visual characterization of cell shape | |
Atomic Force Microscopy | Topographical quantification of cell shape | |
Porosity | Scanning Electron Microscopy | Visual analysis of pore formation |
Motility and mechanics | ||
Cell motility | Manual segmentation of brightfield images | Distance travelled in 24 h before and 24 h after RF treatment |
Elastic Modulus | Atomic Force Microscopy | Cell membrane stiffness |
Cell detachment | Trypan blue assay | Number of cells in supernatant |
Cell adhesion | Atomic Force Microscopy | Number and degree of adhesive sites on the cell membrane |
Cell Proliferation | Manual count of brightfield images and Trypan blue assay | Number of cells adhered to substrate Number of cells in the supernatant |
