Figure 6

Effects of TTX on spexin-induced intestinal and colonic motility.
The jejunum (A) and colon (B) tissues were allowed to equilibrate for 1 hour and TTX was then added into the nutrition buffer. 30 minutes later, the tissues were treated with spexin (1 μM) and the mechanical activities were recorded using the POWERLAB system and CHART5 software. The Emax% of 1 μM spexin in the colon and jejunum were calculated accordingly. Statistical differences between individual groups were evaluated using Student’s t test.