Figure 1 | Scientific Reports

Figure 1

From: Assessing the Efficacy of Mdm2/Mdm4-Inhibiting Stapled Peptides Using Cellular Thermal Shift Assays

Figure 1

Lysate CETSA demonstrated that stapled peptides based on Mdm2-binding site of p53 bind to both Mdm2 and Mdm4.

a,b. Thermal shift plots of lysate CETSA of nutlin-3 and stapled peptide interactions with Mdm2 (a) and Mdm4 (b) and their corresponding ΔTm50 values with 95% confidence intervals, calculated assuming symmetric errors of Tm50s. R2 values of curve fitting are also included. Nutlin-3 and peptides were added to 5 mg/ml AML2 cell lysates at 10 μM.

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