Figure 2 | Scientific Reports

Figure 2

From: Uric Acid Produces an Inflammatory Response through Activation of NF-κB in the Hypothalamus: Implications for the Pathogenesis of Metabolic Disorders

Figure 2

Immunofluorescence analysis of UA-induced astrocyte and microglial accumulation in the rat ARC.

(A) Hypothalamic sections of the rats fed a standard chow (left) or a HUAD (right) were immunostained for GFAP. The image is displayed at × 100 original magnification and the white box indicates the region acquired for the quantification of ARC astrocyte and microglia numbers. (B,C) Immunofluorescence detection of the astrocytic marker GFAP protein (B) and of the microglial marker Iba-1 (C) in coronal sections of the rat hypothalamus (4 μm) from rats fed either chow or a HUAD for 1 day, 7 days, 28 days, 2 months and 3 months. The low-magnification image is × 200 original magnification, which was used for the quantification of ARC astrocyte and microglia numbers. The white box indicates the region presented in the magnification below, which was used to show the activated morphology of the cells. The mean number of ARC astrocytes and microglia in rats fed either chow or a HUAD were quantified in the whole region from the low-magnification view displayed on the lower right (means ± SEM; n = 6 rats per group). *P < 0.05; **P < 0.01; ***P < 0.001 versus chow-fed control.

Back to article page