Figure 1
From: A microfluidic device to study neuronal and motor responses to acute chemical stimuli in zebrafish
Microfluidic device for precise delivery of chemical stimuli.
(a) Setup scheme: an epifluorescence two-photon microscope images neural activity while pulse-like stimuli are delivered to a zebrafish larva. An IR-sensitive camera images tail behavior from below. The microfluidic chip is connected to a computer-controlled manifold for the delivery of two different stimuli, SA and SB. (b) Infrared images of the device around the larva’s face during injection of stimulus SA (infrared dye, left channel). SB (right channel) is buffer. The red rectangle indicates the region used in the kinematic view of Fig. 2a,b (c) Sketch of the fluid flow in the device during the three stationary states: at rest, during injection of SA and during injection of SB. (d) Side-view scheme of the larva’s head resting on the movable slider. The animal’s face is gently cleared before positioning. (e) Scheme of the microfluidic device: the larva’s head embedded in agarose lying on the mobile slider is positioned in the pool in front of the delivery channel. Two electro-microvalves control pressures in stimuli reservoirs SA and SB and trigger injection. p0 is the atmospheric pressure and pin > p0.
