Figure 2

(A) qPCR analysis of the five most likely causative genes in the mouseUC model, namely, EZH2, UPF1, FOXM1, NUDT6 and BAHD1. Bars indicate themean ± SEM;n = 3 per group;*P < 0.05;**P < 0.01. (B) Enrichment plot for UCand BAHD1. The enrichment plot presents the hit position information ofsignature genes in a reference PRL. The size of signature genes is n, andPRL length is N. The function value goes up by 1/n at the hit positions andgoes down by 1/(N-n) at the miss hit positions. The line coloured cyanrepresents the running enrichment score plot of BAHD1 upregulated signaturegenes in UC’s PRL and the final enrichment score (i.e., maximumdeviation of running enrichment score from zero) is 0.0557(). The line coloured yellow represents the runningenrichment score plot of BAHD1 downregulated signature genes inUC’s PRL and the final enrichment score is−0.0706(). The enrichmentscore of BAHD1’s signature in UC’s PRL is. The line coloured blue represents therunning enrichment score plot of UC upregulated signature genes inBAHD1’s PRL and the final enrichment score is0.1738(). The line coloured greenrepresents the running enrichment score plot of UC downregulated signaturegenes in BAHD1’s PRL and the final enrichment score is–0.1331(). The enrichmentscore of UC’s signature in BAHD1’s PRL is. We can infer that the top- (orbottom-) ranked genes in UC are strongly positively (or negatively)regulated in BAHD1, resulting in a high enrichment score forUC’s signature in BAHD1’s PRL.