Figure 4
From: Functional Loss of Bmsei Causes Thermosensitive Epilepsy in Contractile Mutant Silkworm, Bombyx mori
Knockdown of Bmsei with RNAi in WT.
(A) RNAi directed against Bmsei induced the cot phenotype in WT larvae at 42 °C. The larvae showed contraction and rolling. (B) Larvae were injected with Bmsei dsRNA and the cot/cot mutant vomited midgut juice on white paper at 42 °C. WT (no injection) and WT injected with EGFP dsRNA did not display this phenotype. Red arrows show the vomited midgut juices. (C) Expression of Bmsei detected with RT–PCR (Bmsei-q9). (D) Expression of Bmsei normalized to Rpl3 in the qRT–PCR analysis (Bmsei-q9). **P < 0.01, ***P < 0.001. ns, not significant. Error bars depict s.e.m. (n = 3).
