Figure 3

IRGM1 enhances epithelial mesenchymal transition (EMT) of B16 cells.
B16 cells were transduced with IRGM1-GFP lentiviral vectors (a-d, g-i) or tranfected with IRGM1 siRNA to modulate IRGM1 expression (e-f, j-l). (a). EMT morphological change of B16 cells induced by over-expression of IRGM1 were observed by the optical microscope. (b). Microfilaments structure in either control GFP or IRGM1-GFP B16 Cells were detected by transmission electronic microscope (TEM). Phalloidin was used to detect F-actin expression in IRGM1 over-expressing or knocking-down B16 cells and the fluorescence signals were captured by the confocal microscope (c, e) and quantified by flow cytometry (d, f). The expression of E-cadherin and Vimentin was detected by Real-time PCR (g, j) and immunofluroscence staining (h-I, k-l). Data represent at least three independent experiments. (student t test, *p<0.05, **p<0.01)