Table 2 Quantification of dnd gene transcription by quantitative real-time PCR.

From: Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB

 

Average C T  ± SD

 

Bacterium

dndCD

gapA

dndCD fold change relative to gapA *

S. enterica

22.00 ± 0.21

17.76 ± 0.39

1 (0.8–1.1)

dptB −

17.75 ± 0.19

17.43 ± 0.28

15.24 (14.22–16.34)

dptB−(pWHU746)

26.74  ±  0.14

25.63 ± 0.27

0.32 (0.1–1.0)

S. enterica (H2O2, 1mM)

24.84 ± 0.05

23.22 ± 0.18

0.32 (0.27–0.39)

S. enterica (HOCl, 0.4mM)

21.77 ± 0.21

23.67 ± 0.15

0.97 (0.66–1.42)

S. enterica (formaldehyde, 1mM)

21.56 ± 0.38

22.64 ± 0.45

0.77 (0.35–1.71)

  1. Values represent the means ± SD for three analyses.
  2. *The transcriptional changes of dndCD relative to the housekeeping gene, gapA. Quantitative real-time PCR data analysis was performed according to the comparative threshold cycle (CT) method, also known as 2−ΔΔCT.
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