Table 3 Bacterial strains and plasmids.
From: Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB
Strains or plasmids | Relevant properties | Source or reference |
Strains | ||
S. enterica serovar Cerro 87 | strain naturally contains the dndBCDE gene cluster and PT-modified GPSA and GPST | |
dptBˉ | S. enterica derivative, dndB in-frame deletion mutant | |
XTG102 | S. enterica derivative, dndB-E in-frame deletion mutant | |
Plasmids | ||
pBluescript II SK( + ) | cloning vector, Ampr | |
pSJ7 | cloning vector, Ampr | |
pJTU3522 | dndB from S. enterica, cloned in pSJ7 | Gift from Jingdan Liang |
pZWHJ002 | lacZ promoterless, derivative of pXMJ19, Camr | |
pJTU1238 | dndBCDE from S. enterica, cloned in SK(+) | |
pWHU1809 | B1 fragment with promoterless lacZ cloned in pEASY-Blunt Zero (TransGen Biotech) | This study |
pWHU746 | dndB with promoter from S. enterica, cloned in SK(+) | This study |
pWHU751 | pJTU1238 derivative site mutant with C9S | This study |
pWHU752 | pJTU1238 derivative site mutant with C29S | This study |
pWHU753 | pJTU1238 derivative site mutant with C102S | This study |
pWHU754 | pJTU1238 derivative site mutant with C235S | This study |
pWHU755 | pJTU1238 derivative site mutant with C336S | This study |
