Figure 1
Staphylococcus enterotoxin B (SEB) B-cell epitope mapping.
B cell epitope mapping of SEB using an overlapping 18-mer peptide ELISA. To determine the immunodominant peptides of SEB, microtiter plates were coated with synthetic overlapping peptides that spanned the entire length of the SEB of MRSA252 or BSA and OVA192–201 (negative control peptides). Then, sera samples from BALB/c mice that were directly infected with MRSA252 and that were immunised with rSEB plus AlPO4 adjuvant before MRSA252 infection were detected (The antisera were diluted to 1:300). The absorbance was read at 450 nm. The raw O.D. values shown were obtained using serum (diluted by a factor of 1:300) from three independent experiments assayed concurrently. Data are represented as the means ± SEM. Probability values of p < 0.05 were considered significant and are denoted by an asterisk (*). **p < 0.01. (A) Antiserum samples from BALB/c mice immunised with rSEB plus AlPO4 adjuvant following infection with MRSA252 were detected for the immunodominant response. (B) Antiserum samples from non-immunised BALB/c mice infected with MRSA252 were detected for the immunodominant response.
