Figure 1 | Scientific Reports

Figure 1

From: Therapeutic potential of digitoflavone on diabetic nephropathy: nuclear factor erythroid 2-related factor 2-dependent anti-oxidant and anti-inflammatory effect

Figure 1

Digitoflavone activated Nrf2 diminishes mesangial ROS generation under hyperglycemic conditions.

(A) Effects of digitoflavone on ARE-driven luciferase reporter activity in SV40-Mes13 cells. SV40-Mes13 cells were treated with various concentrations of digitoflavone for 24 h. Luciferase activity and cell viability were assayed in parallel as described in the materials and methods section. T-BHQ treatment represented an internal positive control. Top: chemical structure of digitoflavone. (B) and (C) Protein expression of Nrf2 and downstream targets was analyzed in SV40-Mes13 cells incubated in NG with various concentration of digitoflavone (B), HG, or HG + digitoflavone (C) in DMEM media for 24 h. (D) Nrf2 localization was assessed in SV40-Mes13 cells incubated in NG, HG, or HG + digitoflavone DMEM media for 24 h. (E) and (F) Total ROS levels (E) and O2·− levels (F) in SV40-Mes13 cells incubated in NG, HG, or HG + digitoflavone DMEM media are reported. All data represent the mean ± SD of triplicate independent experiments. *P < 0.05 vs. NG group. **P < 0.01 vs. control mice. #P < 0.05 vs. HG group.

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